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Lactic acid Bacteria as biopreservation against spoilage molds in dairy products-a review

The cells were cultured in neurobasal medium (NBM) supplemented with 2% B27, 1% glutamine, epidermal growth factor (EGF, 10 ng/ml), and basic fibroblast growth factor (bFGF, 10 ng/ml)

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Those with conditions like IBS, IBD, gastritis, or ulcers often use oral protocols with doses of 0.5 to 1 mg daily to maximize local gastrointestinal exposure

Cell Adh Migr 9:4882

When fully dissociated, cells were centrifuged (230 g , 3 min) and the cell pellet resuspended and diluted to approximately 2.5E06 cells/mL in Recovery TM Cell Culture Freezing Medium (Gibco, #12648-010)